Structure-Function Biochemistry（Matsushima Lab.）

We have a strong interest in the molecular mechanisms of ligand-receptor interaction. There are many kinds of receptors on cell membranes, in the cytoplasm, in nuclei, etc. When a ligand binds to its specific receptor, it induces a conformational change in the receptor’s structure and the signal is then transmitted. Our main research targets are nuclear receptors which precisely regulate gene transcription. We focus on all nuclear receptors to elucidate their activation mechanisms comprehensively. Binding affinity is analyzed in vitro by radioligand binding assays, and transcription activity is measured by reporter gene assays using cultured cells. The other targets are pain-modulating receptors, which are G-protein couple receptors (GPCRs).